ORDERINGINFORMATION

CatalogNo.:43027

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

TranscriptionfactorJunBisaproteinthatinhumansisencodedbytheJUNBgeneandisinvolvedinregulatinggeneactivityfollowingtheprimarygrowthfactorresponse.ItbindstotheDNAsequence5'-TGA[CG]TCA-3'.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine259(P-B-S(p)-P-I)derivedfromhumanJunBandconjugatedtoKLH.

Accessionno.:P17275,NP_002220.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratJunBonlywhenphosphorylatedatserine259.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofJunB(phospho-Ser259)inextractsofHT29cellsuntreatedortreatedwithEGF.

DetectionofJunB(phospho-Ser259)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.