ORDERINGINFORMATION

CatalogNo.:43028

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

JunDproteinencodedbythisintronlessgeneisamemberoftheJUNfamilyandafunctionalcomponentoftheAP1transcriptionfactorcomplex.Thisproteinhasbeenproposedtoprotectcellsfromp53-dependentsenescenceandapoptosis.Alternativetranslationinitiationsiteusageresultsintheproductionofdifferentisoforms.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine255(G-E-S(p)-P-P)derivedfromhumanJunDandconjugatedtoKLH.

Accessionno.:P17535,NP_005345.3

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratJunDonlywhenphosphorylatedatserine255.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~38kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofJunD(phospho-Ser255)inextractsof293cellswithantibodypre-incubatedwithblockingpeptide(+)orwithoutblockingpeptide(-).

DetectionofJunD(phospho-Ser255)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.