ORDERINGINFORMATION

CatalogNo.:43040

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Theprocessofdifferentiationfrommesodermalprecursorcellstomyoblastshasledtothediscoveryofavarietyoftissue-specificfactorsthatregulatemusclegeneexpressionincludingtheDNAbindingregulatoryproteinmyocyte-specificenhancerfactor-2(MEF2)family.EachoftheseproteinsbindstotheMEF2targetDNAsequencepresentintheregulatoryregionsofmanymuscle-specificgenes.TheMEF2genesaremembersoftheMADSgenefamilythatalsoincludesseveralhomeoticgenesandothertranscriptionfactorsallofwhichshareaconservedDNA-bindingdomain.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine319(V-T-T(p)-P-S)derivedfromhumanMEF2aandconjugatedtoKLH.

Accessionno.:Q02078,NP_001124398.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratMEF2aonlywhenphosphorylatedatthreonine319.

APPLICATION

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofMEF2a(phospho-Thr319)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.