ORDERINGINFORMATION

CatalogNo.:43008

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

MEK2isadualspecificityproteinkinasethatbelongstotheMAPkinasekinasefamily.Thiskinaseplaysanimportantroleinmitogengrowthfactorsignaltransduction.ItphosphorylatesandactivatesMAPK1/ERK2andMAPK2/ERK3.TheactivationofthiskinaseitselfisdependentonSer/ThrphosphorylationbyMAPkinasekinasekinases.TheinhibitionordegradationofthiskinaseisalsofoundtobeinvolvedinthepathogenesisofYersiniaandanthrax.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine(P-G-T(p)-P-T)derivedfromhumanMEK2andconjugatedtoKLH.

Accessionno.:P36507,NP_109587.1

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanMEK2onlywhenphosphorylatedatthreonine394.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~44kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofMEK2(phospho-Thr394)inHepG2andHeLacellextractsuntreatedortreatedwithUV.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofMEK2(phospho-Thr394)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.