ORDERINGINFORMATION

CatalogNo.:43035

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Myc(c-Myc)isaregulatorgenethatcodesfortranscriptionfactorMyc.Thisproteinisamultifunctional,nuclearphosphoproteinthatplaysaroleincellcycleprogression,apoptosisandcellulartransformation.AmutatedversionofMycisfoundinmanycancerswhichcausesMyctobeconstitutivelyexpressed.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine358(R-R-T(p)-H-N)derivedfromhumanMycandconjugatedtoKLH.

Accessionno.:P01106,NP_002458.2

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanMyconlywhenphosphorylatedatthreonine358.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~60kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useat1:100-1:200.

DetectionofMyc(phospho-Thr358)inextractsofHT29cellsuntreatedortreatedwithUV

DetectionofMyc(phospho-Thr358)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofMyc(phospho-Thr358inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.