ORDERINGINFORMATION

CatalogNo.:43016

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

NFκBisatranscriptionfactorfoundinalmostallcelltypesandisinvolvedinmanyBIOLOGicalprocessessuchasinflammation,immunity,differentiation,cellgrowth,tumOrigenesisandapoptosis.NFκBiscontrolledbyvariousmechanismsofpost-translationalmodificationandsubcellularcompartmentalizationaswellasbyinteractionswithothercofactorsorcorepressors.suchasp65-p50andp65-c-Relcomplexes.TheNF-kappa-Bp65-p65complexappearstobeinvolvedininvasin-mediatedactivationofIL-8expression.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine870(Y-G-S(p)-Q-S)derivedfromhumanNFκB-p100andconjugatedtoKLH.

Accessionno.:Q00653,NP_001070962.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratNFκB-p100onlywhenphosphorylatedatserine870.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.

Abandof~120kDaisdetected.

Immunohistochemistry:useatdilutionof1:100-1:200.

Immunofluorescence:useatdilutionof1:100-1:200

DetectionofNFκB-p100(phospho-Ser870)inextractofMDA-MB-435cellsuntreatedortreatedwithTNFα.

DetectionofNFκB-p100(phospho-Ser870)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofNFκB-p100(phosphor-Ser870)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.