ORDERINGINFORMATION

CatalogNo.:43017

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

NFκBisatranscriptionfactorfoundinalmostallcelltypesandisinvolvedinmanyBIOLOGicalprocessessuchasinflammation,immunity,differentiation,cellgrowth,tumOrigenesisandapoptosis.NFκBiscontrolledbyvariousmechanismsofpost-translationalmodificationandsubcellularcompartmentalizationaswellasbyinteractionswithothercofactorsorcorepressors.suchasp65-p50andp65-c-Relcomplexes.TheNF-kappa-Bp65-p65complexappearstobeinvolvedininvasin-mediatedactivationofIL-8expression.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine337(R-K-S(p)-D-L)derivedfromhumanNFκB-p105/p50andconjugatedtoKLH.

Accessionno.:P19838,NP_001158884.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratNFκB-p105/p50onlywhenphosphorylatedatserine337.

APPLICATIONS

Westernblotting:useatdilutionof1:500.Bandsof

~50kDaand~120kDaaredetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useatdilutionof1:100-1:200

DetectionofNFκB-p105/p50(phospho-Ser337)inextractofHeLacells.

DetectionofNFκB-p105/p50(phospho-Ser337)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofNFκB-p105/p50(phospho-Ser337)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.