ORDERINGINFORMATION

CatalogNo.:43014

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

NFκBisatranscriptionfactorfoundinalmostallcelltypesandisinvolvedinmanyBIOLOGicalprocessessuchasinflammation,immunity,differentiation,cellgrowth,tumOrigenesisandapoptosis.NFκBiscontrolledbyvariousmechanismsofpost-translationalmodificationandsubcellularcompartmentalizationaswellasbyinteractionswithothercofactorsorcorepressors.suchasp65-p50andp65-c-Relcomplexes.TheNF-kappa-Bp65-p65complexappearstobeinvolvedininvasin-mediatedactivationofIL-8expression.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine536(F-S-S(p)-I-A)derivedfromhumanNFκB-p65andconjugatedtoKLH.

Accessionno.:Q04206,NP_001138610.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratNFκB-p65onlywhenphosphorylatedatserine536.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.

Abandof~65kDaisdetected.

Immunohistochemistry:useatdilutionof1:100-1:200.

DetectionofNFκB-p65(phospho-Ser536)inHepG2andHL60cellextractsuntreatedortreatedwithTNF.

DetectionofNFκB-p65(phospho-Ser536)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofNFκB-p65(phosphor-Ser536)inparaffin-embeddedhumanlungcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.