ORDERINGINFORMATION

CatalogNo.:43058

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Tumorproteinp73isamemberofthep53familyoftranscriptionfactorsinvolvedincellularresponsestostressanddevelopment.Thefamilymembersincludep53,p63,andp73andhavehighsequencesimilaritytooneanotherwhichallowsp63andp73totransactivatep53-responsivegenescausingcellcyclearrestandapoptosis.Thefamilymemberscaninteractwitheachotherinmanywaysinvolvingdirectorindirectproteininteractions,resultinginregulationofthesametargetgenepromotersorregulationofeachother'spromoters.Thep73proteinisexpressedatverylowlevelsinnormaltissuesandisdifferentiallyexpressedinanumberoftumors.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine99(S-P-Y(p)-A-Q)derivedfromhumanp73.

HostSpecies:Rabbit

Accessionno.:O15350,NP_001119712.1

Specificity:Thisantibodydetectsendogenoushumanp73onlywhenphosphorylatedattyrosine99.

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~80kDaisdetected.

Detectionofp73inextractsof293cellsuntreatedortreatedwithUV.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.