ORDERINGINFORMATION

CatalogNo.:43005

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

PDK1phosphorylatesandactivatesPKB/AKT,PKA,PKC-zeta,RPS6KA1,andRPS6KB1andplaysaroleingeneralsignalingprocessesandindevelopment.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine241(A-N-S(p)-F-V)derivedfromhumanPDK1andconjugatedtoKLH.

Accessionno.:O15530,NP_002604.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectshuman,mouse,andratendogenousPDK1onlywhenphosphorylatedatserine241.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~63kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofPDK1(Phospho-Ser241)in293

andPC12cellextractsuntreatedortreatedwithEGF.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofPDK1(phospho-Ser241)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.