ORDERINGINFORMATION

CatalogNo.:43062

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Phosphataseandtensinhomolog(PTEN)wasidentifiedasatumorsuppressorthatismutatedinmanycancers.PTENisaphosphatidylinositol-3,4,5-trisphosphate3-phosphatase.Itcontainsatensin-likedomainaswellasacatalyticdomainsimilartothedualspecificityproteintyrosinephosphatases.Unlikemostproteintyrosinephosphatases,PTENpreferentiallydephosphorylatesphosphoinositidesubstrates.Itnegativelyregulatesintracellularlevelsofphosphatidylinositol-3,4,5-trisphosphateincellsandfunctionsasatumorsuppressorbynegativelyregulatingAkt/PKBsignalingpathway.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine370(D-V-S(p)-D-N)derivedfromhumanPTENandconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratPTENonlywhenphosphorylatedatserine370.

Accessionno.:P60484,NP_000305.3

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~54kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofPTEN(phospho-Ser370)inextractsofHeLacells.

DetectionofPTEN(phospho-Ser370)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofPTEN(phospho-Ser370)inmethanol-fixedMEFcells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.