ORDERINGINFORMATION

CatalogNo.:43009

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Phosphataseandtensinhomolog(PTEN)wasidentifiedasatumorsuppressorthatismutatedinmanycancers.PTENisaphosphatidylinositol-3,4,5-trisphosphate3-phosphatase.Itcontainsatensin-likedomainaswellasacatalyticdomainsimilartothedualspecificityproteintyrosinephosphatases.Unlikemostproteintyrosinephosphatases,PTENpreferentiallydephosphorylatesphosphoinositidesubstrates.Itnegativelyregulatesintracellularlevelsofphosphatidylinositol-3,4,5-trisphosphateincellsandfunctionsasatumorsuppressorbynegativelyregulatingAkt/PKBsignalingpathway.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine380derivedfromhumanPTENandconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratPTENonlywhenphosphorylatedatserine380.

APPLICATIONS

Immunofluorescence:useatdilutionof1:100-1:200.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofPTEN(phospho-Ser380)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DetectionofPTEN(phospho-Ser380)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITy

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.