ORDERINGINFORMATION

CatalogNo.:43006

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

RAFproto-oncogeneserine/threonine-proteinkinase,alsoknownasRaf1,ispartoftheERK1/2pathwayasaMAPkinasekinasekinase(MAP3K)thatfunctionsdownstreamoftheRassubfamilyofmembraneassociatedGTPases.Raf1isamemberoftheRafkinasefamilyofserine/threonine-specificproteinkinasesfromtheTKL(Tyrosine-kinase-like)groupofkinases.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine259(S-T-S(p)-T-P)derivedfromhumanRaf1andconjugatedtoKLH.

Accessionno.:P04049,NP_002871.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratRaf1onlywhenphosphorylatedatserine259.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~73kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofRaf1(Phospho-Ser259)in293andMDAcellextractsuntreatedortreatedwithPMA.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofRaf1(phospho-Ser259)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.