ORDERINGINFORMATION

CatalogNo.:43020

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Transcriptionfactorc-RelisamemberoftheRel/NFκBfamilywhichalsoincludesRel-A,Rel-B,NFκB1,andNFκB2.TheseproteinsarerelatedthroughahighlyconservedN-terminalregionreferredtoasthe“Reldomain”whichisresponsIBLeforDNAbinding,dimerization,nuclearlocalization,andbindingtotheNFκBinhibitor.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine503(T-S-S(p)-D-S)derivedfromhumanRelandconjugatedtoKLH.

Accessionno.:Q04864,NP_002899.1

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanRelonlywhenphosphorylatedatserine503.

APPLICATION

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofRel(phospho-503)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.