ORDERINGINFORMATION

CatalogNo.:43044

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Signaltransducersandactivatorsoftranscription(STATs)aretranscriptionfactorsthatplayimportantrolesinsignalingpathwaysinducedbymanydifferentcytokinesandgrowthfactorsthatregulatecellgrowthanddifferentiation,theimmuneresponse,antiviralactivity,andhomeostasis.Theiractivationandmechanismsofactionareexamplesofdirectsignalingtothenucleusbyextracellularpolypeptideligandswithouttheinvolvementofsecondmessengers,tyrosinephosphorylationoftranscriptionfactors,ortranscriptionfactorsthatmakedirectcontactwithmembrane-boundreceptorsandDNA.SincethediscoveryofSTAT1and2,thefamilyhasbeenexpandedtosevenmembers.TwoalternativelysplicedisoformsofSTAT1(p84andp91)havebeendescribed.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine701(T-G-Y(p)-I-K)derivedfromhumanSTAT1andconjugatedtoKLH.

Accessionno.:P42224,NP_009330.1

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanandmouseSTAT1onlywhenphosphorylatedattyrosine701.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~91kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofSTAT1(phospho-Tyr701)inextractsofMEFcellstreatedoruntreatedwithIFNγ.

DetectionofSTAT1(phospho-Tyr701)inextractsofHeLacellstreatedwithIFNαorcalfintestinalphosophatase.

DetectionofSTAT1(phospho-Tyr701)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.