ORDERINGINFORMATION

CatalogNo.:43046

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Signaltransducersandactivatorsoftranscription(STATs)aretranscriptionfactorsthatplayimportantrolesinsignalingpathwaysinducedbymanydifferentcytokinesandgrowthfactorsthatregulatecellgrowthanddifferentiation,theimmuneresponse,antiviralactivity,andhomeostasis.Theiractivationandmechanismsofactionareexamplesofdirectsignalingtothenucleusbyextracellularpolypeptideligandswithouttheinvolvementofsecondmessengers,tyrosinephosphorylationoftranscriptionfactors,ortranscriptionfactorsthatmakedirectcontactwithmembrane-boundreceptorsandDNA.SincethediscoveryofSTAT1and2,thefamilyhasbeenexpandedtosevenmembers.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine727(P-M-S(p)-P-R)derivedfromhumanSTAT3andconjugatedtoKLH.

Accessionno.:P40763,NP_003141.2

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratSTAT3onlywhenphosphorylatedatserine727.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~88kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofSTAT3(phospho-Ser727)inextractsofHeLaandHepG2cellsuntreatedortreatedwithPMA.

DetectionofSTAT3(phospho-Ser727)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofSTAT3(phospho-Ser727)inmethanol-fixedHeLacells.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.