ORDERINGINFORMATION

CatalogNo.:43045

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Signaltransducersandactivatorsoftranscription(STATs)aretranscriptionfactorsthatplayimportantrolesinsignalingpathwaysinducedbymanydifferentcytokinesandgrowthfactorsthatregulatecellgrowthanddifferentiation,theimmuneresponse,antiviralactivity,andhomeostasis.Theiractivationandmechanismsofactionareexamplesofdirectsignalingtothenucleusbyextracellularpolypeptideligandswithouttheinvolvementofsecondmessengers,tyrosinephosphorylationoftranscriptionfactors,ortranscriptionfactorsthatmakedirectcontactwithmembrane-boundreceptorsandDNA.SincethediscoveryofSTAT1and2,thefamilyhasbeenexpandedtosevenmembers.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine705(A-P-Y(p)-L-K)derivedfromhumanSTAT3andconjugatedtoKLH.

Accessionno.:P40763,NP_003141.2

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratSTAT3onlywhenphosphorylatedattyrosine705.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~88kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofSTAT3(phospho-Tyr705)inextractsofHeLAcellstreatedoruntreatedwithIFNα.

DetectionofSTAT3(phospho-Tyr705)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofSTAT3(phospho-Tyr705)inparaffin-embeddedhumanlungcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.