ORDERINGINFORMATION

CatalogNo.:43048

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Signaltransducersandactivatorsoftranscription(STATs)aretranscriptionfactorsthatplayimportantrolesinsignalingpathwaysinducedbymanydifferentcytokinesandgrowthfactorsthatregulatecellgrowthanddifferentiation,theimmuneresponse,antiviralactivity,andhomeostasis.Theiractivationandmechanismsofactionareexamplesofdirectsignalingtothenucleusbyextracellularpolypeptideligandswithouttheinvolvementofsecondmessengers,tyrosinephosphorylationoftranscriptionfactors,ortranscriptionfactorsthatmakedirectcontactwithmembrane-boundreceptorsandDNA.SincethediscoveryofSTAT1and2,thefamilyhasbeenexpandedtosevenmembers.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine694(D-G-Y(p)-V-K)derivedfromhumanSTAT5AandconjugatedtoKLH.

Accessionno.:P42229,NP_003143.2

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratSTAT5Aonlywhenphosphorylatedattyrosine694.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~90kDaisdetected.

DetectionofSTAT5A(phospho-Tyr694)inextractsofK562cellsuntreatedortreatedwithG-CSF.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofSTAT5A(phospho-Tyr694)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.