ORDERINGINFORMATION

CatalogNo.:43051

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Signaltransducersandactivatorsoftranscription(STATs)aretranscriptionfactorsthatplayimportantrolesinsignalingpathwaysinducedbymanydifferentcytokinesandgrowthfactorsthatregulatecellgrowthanddifferentiation,theimmuneresponse,antiviralactivity,andhomeostasis.Theiractivationandmechanismsofactionareexamplesofdirectsignalingtothenucleusbyextracellularpolypeptideligandswithouttheinvolvementofsecondmessengers,tyrosinephosphorylationoftranscriptionfactors,ortranscriptionfactorsthatmakedirectcontactwithmembrane-boundreceptorsandDNA.SincethediscoveryofSTAT1and2,thefamilyhasbeenexpandedtosevenmembers.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine645(P-A-T(p)-I-K)derivedfromhumanSTAT6andconjugatedtoKLH.

Accessionno.:P42226,NP_003144.3

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanSTAT6onlywhenphosphorylatedatthreonine645.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~110kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofSTAT6(phospho-Thr645)inextractsofHeLacellstreatedwithIL-4orcalfintestinalphosphatase.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofSTAT6(phospho-Thr645)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.