productdatasheet

CatalogNo.:70104(N3H10)and70105(N9C1).

Size:200ugProteinGpurifiedantibodyinPBS,pH7.4.

Antigen:Proximalsequenceelement(PSE1)ofthehumanU1smallnuclearRNAextractedfromhumanplacenta.HostSpecies:Mouse.

AntibodyClass:70104=IgG2b;70105=IgG1.

Preservatives:None.Availableonrequest.

70104recognizesthesmallβsubunit(~70kDa)and70105recognizesthelargeαsubunit(~80-90kDa)ofplacentalPSE1andhumanKuinsolutionandwhenboundtoDNA.

TheseantibodiesworkwellinWesternblotandelectrophoreticmobility(gelshift)assaysforidentifyingPSE1orKu.Theymaybeusedtoimmuno-depleteKuproteinfromproteinmixturespriortospecificDNAaffinitychromatography.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

KnuthMWetal.1990.JBiolChem

265:17911-17920.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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