productdatasheet

CatalogNo.:70101(8WG16).

Size:200ugProteinGpurifiedantibodyinPBS,pH7.4.

Antigen:PurifiedwheatgermRNApolymeraseII.

HostSpecies:Mouse.

AntibodyClass:IgG2a.Preservatives:None.Availableonrequest.

ThisantibodyreactswiththehighlyconservedheptapeptiderepeatofthelargestsubunitofeukaryoticRNApolymeraseII.Itinhibitspromoter-directedtranscription,butitdoesnotinhibitelongationinthenonspecifictranscriptionassay.

ThisantibodymaybeusedtodetectunproteolyzedRNApolymeraseIIortoinhibitspecifictranscriptionfromclassIIpromoters.Thisisapolyol-responsiveantibodyandcanbeusedingentlepurificationsofRNApolymeraseIIfromfromwheatgerm,calfthymus,andyeastandislikelytopurifyRNApolymeraseIIfrommosteukaryoticorganisms.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

ThompsonNEetal.1989.JBiolChem

264:11511-11520;ThompsonNEetal.

1990.JBiolChem265:7069-7077.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedure

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