CatalogNo.:70102(clone1TBP18).Size:200ugProteinG-purifiedantibodyinPBS,pH7.4.

TheTATA-bindingprotein(TBP)playsacentralroleintheassemblyofmosteukaryotictranscriptioninitiationcomplexes.TBPassembleswithotherproteinstoformuniquemultimericcomplexesforeachofthethreedifferentnuclearRNApolymerases.

TBPhasbeenclonedfromavarietyofspeciesandconsistsoftwodistinctdomains.TheC-terminaldomain(~180aminoacids)

ishighlyconservedamongspecies,whereastheN-terminaldomainvariesconsiderablyinlengthandsequenceamongdifferentspecies.TheconservedC-terminaldomaincontainstheDNA-bindingregionaswellasregionsthatinteractwithpositiveand

negativeregulatoryproteins.InhumanTBP,thenon-conservedN-terminaldomainincludesastretchofglutamineresiduesrangingfrom26to42.SeveralresearchgroupshavefoundanassociationbetweenexpansionofthispolyglutaminetractwithrareformsofspinocerebellarataxiaandotherneurodegenerativediseasesincludingHuntington’sdisease.

Antigen:RecombinanthumanTBP.

HostSpecies:Mouse.

AntibodyClass:IgG1.

Preservatives:None.Availableonrequest.

Thisantibodyrecognizesanepitopewithinaminoacidresidues1-20ofhuman,mouseandratTBP.ItdoesnotreactwithTBPfromDrosophila,yeast,silkworm,orXenopus.Otherspeciesnotinvestigated.

ThisantibodymaybeusedinWesternblotandelectrophoreticmobility(gelshift)assaystodetectmammalianTBP.ItmayalsobeusedtoimmunoprecipitateatruncatedTBPmoleculecontainingonlytheN-terminaldomain.

1. ReactivitywithrhuTBPandHeLacellnuclearextractwithMAb#70102at2ug/ml.

2. EpitopemappingbyimmunoblotwithfragmentsofrhuTBPwithMAB#70102at2ug/ml.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

ThompsonNEetal.2004ProteinExpressionandPurification36:186-197.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.