productdatasheet
CatalogNo.:55101thru55105Size:100ugProteinG-purifiedantibodyinPBS,pH7.4.
Antigen:Mixtureofnativeandformaldehyde-zincfixedspermpurifiedthroughaPercollgrADIent.
HostSpecies:MouseAntibodyClass:55105=IgG3;55101thru55104=IgG1.
StABIlizers:NonePreservatives:None.Availableonrequest.
Theseantibodiesspecificallyrecognizemousespermproteinsp56.Theywillreactwithdenatured,nonreducedsp56butnotwithdenatured,reducedsp56.Theseantibodiesdonotcross-reactwithhumansperm.Relativeantibodytitersforantibodybindingtosolidphasesp56:55101(7C5)=66pM,55102(5F12)=4nM,55103(4B2)=29nM,55104(7H12)=70nM,55105(12D2)
=400nM.
APPLICATIONSTheseantibodiesmaybeusedinimmunohistochemistrytolocalizesp56onmousespermandinimmunoblotsorELISAtoidentifysp56.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Chengetal.(1994)JCellBiol125:867;
Bookbinderetal.(1995)Science269:
86.
Forinvitroinvestigationaluseonly.
Notforuseintherapeuticordiagnosticprocedures.
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