
ChlorideChannelProtein3(Clcn3)MonoclonalAntibody
IonchannelsareintegralmembraneproteinsthathelpestablishandcontrolthesmallvoltagegrADIentacrosstheplasmamembraneoflivingcellsbyallowingtheflowofionsdowntheirelectRochemicalgradient.Clcn3mediatestheexchangeofchlorideionsagainstprotonsandfunctionsasanantiporter,contributingtotheacidificationoftheendosomeandsynapticvesiclelumen.ItmayplayanimportantroleinneuronalcellfunctionthroughregulationofmembraneexcitABIlitybyproteinkinaseC.
Thisantibodyrecognizeshuman,mouse,andratClcn3.Itdoesnotcross-reactwithClcn4orClcn5.
APPLICATIONSImmunoblotting:useat1-2ug/ml.Abandof~90kDaisdetected.
Immunohistochemistry:useat1-10ug/ml.Thesearerecommendedconcentrations.Usershoulddetermineoptimal
concentrationsfortheirapplication.
Positivecontrol:Ratbrainmembranes.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.