ORDERINGINFORMATION
CatalogNo. | CloneNo. | MAbSubtype | Size | LibraryPackNo. | 100ug/clone |
10101 | F82.2.9 | IgG1 | 100ug,500ug | 101101 | All4clones |
10102 | F82.2.32 | IgG1 | 100ug,500ug | ||
10103 | F85.5.72 | IgG1 | 100ug,500ug | ||
10104 | F8C27.4 | IgG2a | 100ug,500ug |
Format:ProteinG-purifiedantibodyinPBS,pH7.4.
BACKGROUND
FactorVIII(FVIII)isanessentialblood-clottingprotein,alsoknownasanti-hemophilicfactor(AHF).Inhumans,factorVIIIisencodedbytheF8gene.DefectsinthisgeneresultinhemophiliaA,arecessiveX-linkedcoagulationdisorder.FactorVIIIisproducedinliversinusoidalcellsandendothelialcellsoutsideoftheliverthroughoutthebody.Thisproteincirculatesinthebloodstreaminaninactiveform,boundtoanothermoleculecalledvonWillebrandfactor,untilaninjurythatdamagesbloodvesselsoccurs.Inresponsetoinjury,factorVIIIisactivatedandseparatesfromvonWillebrandfactor.Theactiveprotein(sometimeswrittenasfactorVIIIa)interactswithanothercoagulationfactorcalledfactorIX.Thisinteractionsetsoffachainofadditionalchemicalreactionsthatformabloodclot.
SPECIFICATIONSUMMARY
Antigen:PurifiedhumanFactorVIII.
GeneID:2157
Accessionnos.:NP_000123,P00451
HostSpecies:Mouse
Specificity:10101,10102,and10103recognizefull-lengthhumanFactorVIII(epitopesnotdetermined).10104recognizesanepitopeattheN-terminalregionofthe83kDalightchainofFactorVIII.Theseantibodiesdonotcross-reactwithvonWillebrandfactor.
APPLICATIONS
TheseantibodieshavebeenqualifiedforuseinELISAandimmunoblottingtodetecthumanFactorVIII.
#10104DetectionofFactorVIIIlightchain(LCh)inwild-type(WT)andrecombinant(R372H)FactorVIIIafterreactionwiththrombinovertime(Nogamietal.2005Blood105:4362-4368).
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
REFERENCESFOR#10104
TakeyamaMetal.(2012)Biochemistry51:820-828.
NogamiKetal.(2005)Blood105:4362-4368.
FayPJetal.(2001)JBiolChem276:12434-12439.
FayPJ(1988)ArchBiochemBiophys262:525-531.FayPJetal.(1986)BiochimBiophysActa87:268-278.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
