ORDERINGINFORMATION
Catalognos.13001P-55ug13001P-2525ug13001P-10001000ugFormulation:Sterile-filteredsolutionin20mMTris-HCl,pH7.5and10%glycerol.Purifiedbyproprietarychromatographictechniques.BACKGROUND
Cu/ZnSuperoxideDismutase(SOD1)catalyzesthereactionbetweensuperoxideanionsandhydrogentoyieldmolecularoxygenandhydrogenperoxide.Itprotectsacellfromdangerouslevelsofsuperoxide.SOD1bindscopperandzincionsandisoneofthreeisozymesresponsIBLefordestroyingfreesuperoxiderADIcals.MutationsinSOD1causeaformoffamilialamyotrophiclateralsclerosis(ALS).
DESCRIPTION
RecombinantHumanCu/ZnSOD1producedinE.coliisasinglemonomericnon-glycosylatedpolypeptidechaincontaining154aminoacidswithamolecularweightof15.9kDa.
SPECIFICATIONSUMMARY
Source:EscherichiacoliPurity:Greaterthan95%asdeterminedbySDS-PAGEandRP-HPLC.Accessionnumber:P00441.2Aminoacidsequence:MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
BIOLOGICALACTIVITY
Specificactivityis>90units/mginwhichoneunitwillinhibittherateofreductionofcytochromecby50%inacoupledsystemusingxanthineandxanthineoxidaseatpH7.8at25oCina1.5mlreactionvolume.
STORAGEANDSTABILITY
Storeat4oCifentirevialwillbeusedwithin2-4weeks.Storeat-20oCforlongerperiodsoftime.Additionofacarrierprotein(0.1%HSAorBSA)isrecommendedforlong-termstorage.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
