productdatasheet
CatalogNumber | Size |
11116P-10 | 10ug |
11116P-50 | 50ug |
11116P-1000 | 1000ug |
CatalogNumber | Size |
11116P-10 | 10ug |
11116P-50 | 50ug |
11116P-1000 | 1000ug |
ORDERINGINFORMATION
Formulation:Sterile-filteredcolorlesssolutioncontains20mMTris,pH7.5,and2mMDTThavingaproteinconcentrationof1mg/ml.Purifiedbyproprietarychromatographictechniques.
Hsp70playsanimportantroleinproteinfoldingandinprotectingcellsfromstress.InmostspeciestherearemanyproteinsthatbelongtotheHsp70family.Someareexpressedonlyunderstressconditionswhileothersarepresentincellsundernormalconditionsandarenotheat-inducIBLe.
RecombinantHumanDnaK(Hsp70)producedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining661aminoacidsfusedtoHis-tagattheN-terminuswithatotalmolecularweightof72.2kDa.
Source:Escherichiacoli
Purity:Greaterthan95%asdeterminedbySDS-PAGEandRP-HPLC.
Accessionnumber:P08107
AminoAcidSequence:
MGSSHHHHHHSSGLVPRGSHMAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGDTKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD
Althoughstableat4oCfor4weeks,productshouldbestoredatorbelow
-20oC.Forlongtermstorage,addition
ofacarrierprotein(0.1%HSAorBSA)isrecommended.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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