ORDERINGINFORMATION
Catalognos.:13003P-55ug13003P-2525ug13003P-10001000ugFormulation:Sterile-filteredsolutionin20mMTris-HCl,pH8.0,20%glycerol.Purifiedbystandardchromatographictechniques.BACKGROUND
SOD2ispartoftheiron/manganesesuperoxidedismutasefamily.Itencodesamitochondrialproteinthatformsahomotetramerandbindsonemanganeseionpersubunit.SOD2bindstothesuperoxidebyproductsofoxidativephosphorylationandconvertsthemtohydrogenperoxideanddiatomicoxygen.MutationsinSOD2genehavebeenassociatedwithidiopathiccardiomyopathy(IDC),prematureaging,sporADIcmotorneurondisease,andcancer.SOD2destroysradicalswhichareusuallyproducedwithinthecellsandwhicharetoxictoBIOLOGicalsystems.
DESCRIPTION
RecombinantHumanMnSOD2producedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining219aminoacids(aa25-222)withamolecularweightof24.4kDa.IthasaHis-tagattheN-terminus.
SPECIFICATIONSUMMARY
Source:EscherichiacoliPurity:Greaterthan95%asdeterminedbySDS-PAGE.Accessionnumber:P04179.2Aminoacidsequence:MGSSHHHHHHSSGLVPRGSHMKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWTNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYMACKK.
BIOLOGICALACTIVITY
Specificactivityis>1,200units/mginwhichoneunitwillinhibittherateofreductionofcytochromecby50%inacoupledsystemusingxanthineandxanthineoxidaseatpH7.8at25oCina1.5mlreactionvolume.
STORAGEANDSTABILITY
Storeat4oCifentirevialwillbeusedwithin2-4weeks.Storeat-20oCforlongerperiodsoftime.Additionofacarrierprotein(0.1%HSAorBSA)isrecommendedforlong-termstorage.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
