12205P-1010ug
12205P-5050ug
12205P-10001000ug
Format:Sterilefiltered,lyophilizedfroma1mg/mlsolutionin10mMsodiumphosphatebuffer,pH8.0and50mMNaCl.Purification:Chromatographic.DESCRIPTION
HumanRecombinantProlactinproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining200aminoacidsandhavingamolecularmassof23007Dalton.ThesequenceofthefirstfiveN-terminalaminoacidsisMet-Leu-Pro-Ile-Cys.Accessionno.P01236.
SPECIFICATIONSUMMARY
Source:EscherichiacoliStABIlizers:NonePreservatives:NonePurity:Greaterthan95%bySEC-HPLCandSDS-PAGE.BIOLOGicalActivity:ED50determinedbydose-dependentstimulationoftheproliferationofratlymphomacellsNb2-11<0.065ng/mlcorrespondingtoaSpecificActivityof15,385,000IU/mg.SOLUBILITY
ReconstitutelyophilizedRecombinantHumanProlactininsterileH2Otonotlessthan100ug/ml.Canbedilutedfurtherinotheraqueoussolution.
STORAGEANDSTABILITY
Althoughstableatroomtemperaturefor3weeks,productisbeststoreddesiccatedbelow-18oC.Followingreconstitution,productcanbestoredat4oCfor2-7days.Forlongtermstorage,reconstitutedproductisbeststoredat-18oCwiththeadditionofastabilizingproteinsuchas0.1%HSAorBSA.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
