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QED Bioscience/Recombinant Human Thyroid Peroxidase (TPO)/25004P/1 Ea
ADVANCEDRESEARCHTECHNOLOGIES
CatalogNumber | Size |
25004P | 100ug |
Formulation:Lyophilizedfrom20mMTris-HCl,pH8.0,250mMNaCl,0.1mMKI,5%D-mannitol.ReconstitutewithsteriledeionizedH2O.
BACKGROUND
Thyroidperoxidase(TPO)isanintegralapicalmembraneglycoproteinofthyroidfollicularcellsandplaysakeyroleinthyroidhormonebiosynthesis.Itisahomodimercomprisedoftwoidenticalsubunitsofapprox.100kDa.TPOisoneofthemainautoantigenictargetsinautoimmunethyroiddiseaseofhumans.Anti-TPOautoantibodiesarepresentin90%ofcasesofHashimoto’sthyroiditisand75%ofcasesofGraves’disease.
DESCRIPTION
RecombinanthumanTPOproducedininsectcellscorrespondstoaa19-846(calculatedmolecularweightapprox.
92kDa)ofthesolubleextracellulardomainofhumanTPO.Itdoesnotcontainanytags.Itispurifiedbyspecificmonoclonalantibody-basedaffinityandionexchangechromato-graphicmethods.InSDS-PAGE,twobandsintheregionof100-110kDaareobservedduetodifferentialglycosyl-ationoftheprotein.ImmunoreactivityofrhuTPOisconfirmedbyELISAwithpatientserumsamplescontaining
anti-TPOautoantibodies.
SPECIFICATIONSUMMARY
Source:Insectcells
Purity:Greaterthan95%asdeterminedbySDS-PAGE.IdentityconfirmedbyMALDImassspectrometry.
SDS-PAGEofrecombinanthumanTPO,reducingconditions.
Lane1:MWstandards250,130,100,70,
55,35,25,15,and10kDa.
Lane2:recombinanthumanTPO,1ug.
STORAGEANDSTABILITY
Storeat20oCinlyophilizedform.Afterreconstitution,storeat-70oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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