CatalogNo.Size

50186P-22ug

50186P-1010ug

50186P-5050ug

50186P-100100ug

50186P-10001000ug

CCL2,alsoknownasMonocyteChemoattractantProtein-1(MCP-1)isasmallcytokine(~13kDa)thatbelongstotheCCchemokinefamily.CCL2recruitsmonocytes,memoryTcells,anddendriticcellstositesofinflammationproducedbyeithertissueinjuryorinfection.Itisanchoredintheplasmamembraneofendothelialcellsbyglycosaminoglycansidechainsofproteoglycans.Tobecomeactivated,CCL2proteinhastobecleavedbymetalloproteinaseMMP-12.CCL2isimplicatedinthepathogenesisofseveraldiseasescharacterizedbymonocyticinfiltrates,suchaspsoriasis,rheumatoidarthritisandatherosclerosis.CCR2andCCR4aretwocellsurfacereceptorsthatbindCCL2.

Source:RecombinanthumanCCL2isproducedinE.coli(accessionno.

P13500).

ProteinSequence:QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT

MolecularMass:8.662kDabyMassSpec.

Purity:>97%bySDS-PAGE

Activity:EC50=0.067nMdeterminedbymigrationorrecombinantCCR2-expressingcells.

EndotoxinLevel:<0.01EUper1ugofproteinbyLALmethod.

Form:Lyophilized.

CarrierProtein:None.

Reconstitution:Recommendedat100ug/mlinsteriledistilledwater.

StABIlityandStorage:12monthsfromdateofreceipt,-20oCto-70oC,assupplied.

1month,2oCto8oC,understerileconditionsafterreconstitution.

3months,-20oCto-70oC,understerileconditionsafterreconstitution.

Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.