CatalogNo.Size
50186PB-22ug
50186PB-1010ug
50186PB-5050ug
50186PB-100100ug
CCL2,alsoknownasMonocyteChemoattractantProtein-1(MCP-1)isasmallcytokine(~13kDa)thatbelongstotheCCchemokinefamily.CCL2recruitsmonocytes,memoryTcells,anddendriticcellstositesofinflammationproducedbyeithertissueinjuryorinfection.Itisanchoredintheplasmamembraneofendothelialcellsbyglycosaminoglycansidechainsofproteoglycans.Tobecomeactivated,CCL2proteinhastobecleavedbymetalloproteinaseMMP-12.CCL2isimplicatedinthepathogenesisofseveraldiseasescharacterizedbymonocyticinfiltrates,suchaspsoriasis,rheumatoidarthritisandatherosclerosis.CCR2andCCR4aretwocellsurfacereceptorsthatbindCCL2.
Source:RecombinanthumanCCL2isproducedinE.coli(accessionno.
P13500,aa24-99).
Modification:Biotinylated
ProteinSequence:QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT
MolecularMass:11.084kDabyMassSpec.
Purity:>97%bySDS-PAGE
Activity:EC50=0.25-1.2nMdeterminedbyMigrationAssayofrecombinantCCR2-expressingcells.
EndotoxinLevel:<0.01EUper1ugofproteinbyLALmethod.
Form:Lyophilized.
CarrierProtein:None.
Reconstitution:CentrifugetubepriortoresUSPending.Recommendedat100ug/mlinsteriledistilledwater.
StABIlityandStorage:12monthsfromdateofreceipt,-20oCto-70oC,assupplied.
1month,-20oCto-70oC,understerileconditionsafterreconstitution.Bestifusedimmediatelyafterreconstitution.
Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.
