CatalogNo.Size

50190PB-22ug

50190PB-1010ug

50190PB-5050ug

50190PB-100100ug

ThesmallcytokineCXCL8(alsoknownasIL-8)isknowntobeoneofthemostpotentchemoattractantmoleculesthat,amongseveralotherfunctions,isresponsIBLeforguidingneutrophilsthroughthetissuematrixuntiltheyreachsitesofinjury.IL-8isalsoapotentpromoterofangiogenesis.Intargetcells,IL-8bindstotwocellsurfacereceptors,CXCR1andCXCR2,and

inducesaseriesofphysiologicalresponsesrequiredformigrationandphagocytosis,suchasincreaseofintracellularCa2+,exocytosis(e.g.histaminerelease),andrespiratoryburst.IL-8isamemberoftheCXCchemokinefamily.Thegenesencodingthisandtheothertenmembersof

theCXCchemokinefamilyformaclusterinaregionmappedtochromosome4q.

Source:RecombinanthumanCXCL8isproducedinE.coli(accessionno.

P10145,aa28-99).

Modification:Biotinylated

ProteinSequence:SAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS

MolecularMass:10.8kDabyMassSpec.

Purity:>97%bySDS-PAGE

Activity:EC50=0.5-1.0nMdeterminedbyMigrationAssayofrecombinantCXCR1-expressingcells.

EndotoxinLevel:<0.01EUper1ugofproteinbyLALmethod.

Form:Lyophilized.

CarrierProtein:None.

Reconstitution:Recommendedat100ug/mlinsteriledistilledwater.

StABIlityandStorage:12monthsfromdateofreceipt,-20oCto-70oC,assupplied.

1month,-20oCto-70oC,understerileconditionsafterreconstitution.Bestifusedimmediatelyafterreconstitution.

Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.