product data sheet

Yersinia pestis V Antigen Monoclonal Antibodies

Catalog Number

Size

18772-200

200ug

18773-200

200ug

18774-200

200ug

18775-200

200ug

18776-200

200ug

18772-1000

1mg

18773-1000

1mg

18774-1000

1mg

18775-1000

1mg

18776-1000

1mg

Catalog Number

Size

18772-200

200ug

18773-200

200ug

18774-200

200ug

18775-200

200ug

18776-200

200ug

18772-1000

1mg

18773-1000

1mg

18774-1000

1mg

18775-1000

1mg

18776-1000

1mg

ORDERING INFORMATION

Format: These antibodies are purified by Protein G affinity chromatography and are supplied in PBS, pH 7.4, containing 0.1% sodium azide as preservative.

Y. pestis, the causative agent of plague, has low-Ca+2 response (LCR) virulence plasmids that encode a set of secreted virulence proteins including Yersinia outer proteins (YOPS) and the V antigen, LcrV. Some of the YOPS play a role in adherence to eukaryotic cells while others are targeted into a eukaryotic cell at the site of adherence. The YOPS derange cellular signaling and cytoskeletal functions necessary for host defenses such as phagocytosis. LcrV is a secreted anti-host component with direct immunomodulatory effects. LcrV expression is involved in targeting of YOPS to host cells and is required for cytotoxicity.

Antigen: Recombinant full-length

Y. pestis V antigen.

Host Species: Mouse Antibody Class: All are IgG1. Preservative: 0.1% NaN3

These antibodies specifically recognize

Y. pestis V antigen. They do not cross- react with Y. pestis capsular F1 antigen.

Immunoblotting: End-user should determine optimal concentration. We recommend testing a concentration range of 1-10ug/ml. A band of 37 kDa is detected.

ELISA: End-user should determine optimal concentration for their sample. Sandwich ELISA: use 18772 as coating antibody and 18773 as detect antibody.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one

(1) year at 4oC or -20oC. Avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

QED Bioscience, Inc. 10919 Technology Place, Suite C

San Diego, CA 92127

Toll Free 800.929.2114

Phone 858.675.2405

Fax 858.592.1509

info@qedbio.com

Visit our website for additional product information and to order online.

qedbio.com