Catalog No.

15301

Clone No.

GNE13-337.5

MAb Subtype

IgG2b*

Size

100ug, 500ug

Library Pack No.

153101

100ug/clone

All 9 clones

15302

GNE13-305.6

IgG2a*

100ug, 500ug

15303

GNE10-113.3

IgG3**

100ug, 500ug

15304

GNE11-265.5

IgG3**

100ug, 500ug

15305

GNE11-270.3.1

IgG2a*

100ug, 500ug

15306

GNE11-222.4

IgM**

100ug, 500ug

15307

HEC10-172.5

IgM**

100ug, 500ug

15308

HEC11-445.2

IgM**

100ug, 500ug

15309

IVEC9-220.1

IgM**

100ug, 500ug

Catalog No.

15301

Clone No.

GNE13-337.5

MAb Subtype

IgG2b*

Size

100ug, 500ug

Library Pack No.

153101

100ug/clone

All 9 clones

15302

GNE13-305.6

IgG2a*

100ug, 500ug

15303

GNE10-113.3

IgG3**

100ug, 500ug

15304

GNE11-265.5

IgG3**

100ug, 500ug

15305

GNE11-270.3.1

IgG2a*

100ug, 500ug

15306

GNE11-222.4

IgM**

100ug, 500ug

15307

HEC10-172.5

IgM**

100ug, 500ug

15308

HEC11-445.2

IgM**

100ug, 500ug

15309

IVEC9-220.1

IgM**

100ug, 500ug

ORDERING INFORMATION

**PEG-purified antibody in PBS, pH 7.4

BACKGROUND

Endotoxin is the lipopolysaccharide (LPS) complex associated with the outer membrane of Gram- negative bacteria such as Escherichia coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus influenzae, Bordetella pertussis and Vibrio cholerae. Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of Gram-negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses, and it activates complement by the alternative (properdin) pathway.

SPECIFICATION SUMMARY

Host Species:

Mouse

Catalog No.

Reactivity

Reactivity Key

15301

2, 3, 4, 5

2. E. coli J5 LPS

15302

2,4,5

3. Klebsiella pneumoniae

15303

2

4. Shigella sonnei

15304

2,4

5. Salmonella typhimurium

15305

2, 3, 4, 5

6. Enterobacter aerogenes

15306

2, 4, 5, 7, 8, 9, 10

7. Serratia marcescens

15307

2, 3, 7, 8, 9, 11

8. Proteus mirabilis

15308

2, 6, 7, 8, 10, 11

9. Proteus vulgaris

15309

2, 3, 6, 7, 8, 9, 10, 11

10. Acinetobacter calcoaceticus

11. Pseudomonas aeruginosa

APPLICATIONS

These antibodies have been qualified for use in ELISA to detect gram-negative endotoxin and in dot-blots to detect cells that express gram negative endotoxin. Endusers should determine optimal concentrations for their applications.

Customer feedback: #15305 was conjugated to colloidal gold and used successfully in a lateral flow assay format.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20o to -70oC. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.